B گالاکتوسیداز g5635 سیگماβ-Galactosidase from Escherichia coli g5635 sigma
B گالاکتوسیداز g5635 سیگما
-Galactosidase from Escherichia coli g5635 sigma
G5635SIGMA -Galactosidase fromEscherichia coli Grade VIII, lyophilized powder, 500units/mg protein
دسته بندی های مرتبط 3.2.x.x گلیکوزیدازها ، 3.x.x.x هیدرولازها ، شاخص کاربرد ، بیوشیمیایی و واکنش دهنده ها ، هیدرولیز کربوهیدرات ،
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سطح کیفیت PREMIUM
نوع درجه هشتم
ایجاد پودر لیوفیلیزه
شامل BSA به عنوان توسعه دهنده نیست
ترکیب پروتئین ، 60 bi بیورت
دمای ذخیره سازی 20 درجه سانتی گراد
Properties
Related Categories 3.2.x.x Glycosidases,3.x.x.x Hydrolases,Application Index,Biochemicals and Reagents,Carbohydrate Hydrolysis,
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Quality Level PREMIUM
type Grade VIII
form lyophilized powder
does not contain BSA as extender
composition Protein, 60% biuret
storage temp. 20C
Description
Packaging
1000, 3000, 5000, 15000 units in serum bottle Unit Definition
One unit will hydrolyze 1.0 mole of o-nitrophenyl -D-galactoside to o-nitrophenol andD-galactose per min at pH 7.3 at 37 C. Physical form
Contains Tris buffer salts and magnesium chloride Application
-Galactosidase is conjugated to an antibody which specifically recognizes a target molecule (enzyme immunoassay or EIA).[1]-Galactosidase is also used as a reporter enzyme to monitor the level of gene expression of a promoter.[2]It may be used as a positive control protein with anti--galactosidase antibodies. Biochem/physiol Actions
-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of -galactosidase, in a feedback loop. General description
-Galactosidase is a tetramer consisting of four equal subunits of 135,000 each. It is a sulfhydryl containing enzyme, with 19 cysteine residues per subunit.[3] Physical properties
Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)
Safety Information
Personal Protective Equipment
Eyeshields,Gloves,type N95 (US),type P1 (EN143) respirator filter
RIDADR
NONH for all modes of transport
WGK Germany
3
RTECS
LW5840000