RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas
سرم خون جنین گاو محصول جیبکو Description
RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.
Unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins
Contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium
Vitamins inositol and choline are present in high concentrations
Contains GlutaMAX supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format
Modified as follows: with GlutaMAX(tm), phenol red; without HEPES. RPMI 1640 medium contains no proteins, lipids, or growth factors
Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
Uses sodium bicarbonate buffer system (2.0g/L) and therefore requires a 5-10% CO2environment to maintain physiological pH
Sustainable packaging
Cell Culture, Mammalian Cell Culture
Specifications
Format Liquid
Formulation Biotin, Calcium nitrate (Ca(NO3)2 4H2O), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Folic Acid, Glutathione (reduced), Glycine, L-Alanyl-Glutamine, L-Arginine, L-Asparagine, L-Aspartic acid, L-Cystine, L-Glutamic Acid, L-Histidine, L-Hydroxyproline, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine, L-Valine, Magnesium Sulfate (MgSO4-7H2O), Niacinamide, Para-Aminobenzoic Acid, Phenol Red, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate dibasic (Na2HPO4) anhydrous, Thiamine hydrochloride, Vitamin B12, i-Inositol
Storage Requirements Storage: 2-8 C. Protect from light. Shelf life: 12 months from date of manufacture
With Additives No Sodium Pyruvate; GlutaMAX(tm)-I; Phenol Red
Packaging Sustainable packaging